Education

Harvard University | Cambridge, MA
Ph.D. in Biochemistry (in progress) | 2019 - Present

Yale University | New Haven, CT
B.S. in Molecular Biophysics & Biochemistry | 2015-2019

Experience

Lab of Prof. Doeke Hekstra, Harvard University | Graduate Researcher | 2019 - present
Investigation of protein structure and dynamics via electric-field stimulated crystallography (EF-X) and other crystallographic methods

Lab of Prof. José María Delfino, Universidad de Buenos Aires | Visiting Researcher | Summer 2019
Probing of protein solvent-accessible surface area via diazarine photochemical labelling while practicing Spanish-language immersion

Lab of Prof. Patrick Loria, Yale University | Undergraduate Researcher | 2018 - 2019
Biophysical and biochemical characterization of protein tyrosine phosphatase structure and dynamics via NMR and crystallography

Merck & Co. | Structural Science Intern | Summer 2017
Crystal structure determination of a GPCR in complex with drug-candidate ligands

Lab of Prof. Craig Crews, Yale University | Undergraduate Researcher | 2016 - 2017
Investigation of cellular responses to partial protein unfolding through a variety of methods

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Publications

Laue-DIALS: open-source software for polychromatic X-ray diffraction data, Structural Dynamics, 2024

MatchMaps: non-isomorphous difference maps for X-ray crystallography, J. Appl. Cryst., 2024

Perturbative diffraction methods resolve a conformational switch that facilitates a two-step enzymatic mechanism, PNAS, 2024

Uncovering the Molecular Interactions in the Catalytic Loop That Modulate the Conformational Dynamics in Protein Tyrosine Phosphatase 1B, JACS, 2019

Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response, MBoC, 2018

Skills

X-Ray Crystallography

• Protein crystallization and screen design using vapor diffusion, lipidic cubic phase methods
• DIALS, CCP4, PHENIX, and Coot for data processing and structure determination
• Modeling of multiple loop states and unknown ligands
• Customized handling of unusual diffraction data via reciprocalspaceship
• Phasing via selenomethionine labelling and single-wavelength anomalous dispersion

Biochemistry & Biophysics

• Protein expression and purification (IMAC, IEX, SEC) using FPLC and manual methods
• Enzyme kinetics assays in both steady-state and pre-steady-state regimes
• Immunoprecipitation (pulldown) to determine protein binding partners
• Protein stability quantification via limited proteolysis, melting-point analysis, colorometric assays
• Diazarine labelling for analysis of solvent-accessible surface area
• Two-dimensional ¹H ¹³C HSQC NMR for investigation of enzyme dynamics
• One-dimensional ³¹P NMR for study of phosphoenzyme species

Cell & Molecular Biology

• DNA cloning for creation of novel protein constructs including fusion proteins and point mutants
• Mammalian tissue culture including transfection of plasmids of interest
• Western blot analysis of protein levels in cell lysate
• Confocal microscopy to assess subcellular localization of fluorescent fusion proteins

Programming

• Python, bash, R, Java

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